Sirtuin 6 Fluorogenic Assay Kit

The Sirtuin 6 Fluorogenic Assay Kit is a complete assay system designed to measure SIRT6 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT6 activity measurements. In addition, the kit includes purified SIRT6 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT6 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT6 activity level. First, the Sirtuin 6 fluorometric substrate, containing a myristoylated lysine side chain, is incubated with purified SIRT6 enzyme. The demyristoylation sensitizes the substrate so subsequent treatment with the SIRT Developer produces a fluorophore that can then be measured using a fluorescence reader.