SIRT3 (Sirtuin3) Fluorogenic Assay Kit

The Fluorogenic SIRT3 Assay Kit is a complete assay system_x000D_designed to measure Sirtuin 3 (SIRT3) activity for screening and profiling_x000D_applications. The Fluorogenic SIRT3 Assay Kit is based on_x000D_a unique fluorogenic substrate and developer combination. This assay method_x000D_eliminates dealing with the radioactivity, extraction, and chromatography aspects_x000D_of traditional assays. Using this kit, only two simple steps on a microtiter plate are_x000D_needed to analyze the SIRT3 activity level. First, the HDAC fluorometric_x000D_substrate (HDAC substrate 1), containing an acetylated lysine side chain, is_x000D_incubated with purified SIRT3 enzyme. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can_x000D_then be measured using a fluorescence reader.