SIRT1 (Sirtuin1) Fluorogenic Assay Kit

The Fluorogenic SIRT1 Assay Kit is a complete assay system_x000D_ designed to measure Sirtuin 1 (SIRT1) activity for screening and profiling_x000D_applications. The Fluorogenic SIRT1_x000D_ Assay Kit is based on a unique fluorogenic substrate and developer combination._x000D_ This assay method eliminates dealing with the radioactivity, extraction, and_x000D_chromatography aspects of traditional assays. Using this kit, only two simple_x000D_ steps on a microtiter plate are needed to analyze the SIRT1 activity level. First,_x000D_ the SIRT fluorometric substrate (SIRT substrate 1), containing an acetylated_x000D_ lysine side chain, is incubated with purified SIRT1 enzyme. The deacetylation_x000D_ sensitizes the substrate so subsequent treatment with the Lysine Developer_x000D_ produces a fluorophore that can then be measured using a fluorescence reader.