SIRT2 (Sirtuin2) Fluorogenic Assay Kit

The Fluorogenic SIRT2 Assay Kit is a complete assay system_x000D_ designed to measure Sirtuin 2 (SIRT2) activity for screening and profiling_x000D_ applications. It comes in a convenient 96-well format, with all the reagents_x000D_ necessary for 32 fluorescent SIRT2 activity measurements*.The kit_x000D_ includes purified SIRT2 enzyme and a SIRT inhibitor, Nicotinamide, for use as a_x000D_ positive and negative control, respectively. The Fluorogenic SIRT2 Assay Kit is_x000D_ based on a unique fluorogenic substrate and developer combination. This assay_x000D_method eliminates dealing with the radioactivity, extraction, and chromatography_x000D_ aspects of traditional assays. Using this kit, only two simple steps on a microtiter_x000D_plate are needed to analyze the SIRT2 activity level. First, the SIRT fluorometric_x000D_ substrate (SIRT substrate 1), containing an acetylated lysine side chain, is_x000D_incubated with purified SIRT2 enzyme. The deacetylation sensitizes the substrate so_x000D_ subsequent treatment with the Lysine Developer produces a fluorophore that can_x000D_then be measured using a fluorescence reader._x000D_