ADAMTS4 Fluorogenic Assay Kit

The ADAMTS4 Fluorogenic Assay Kit is designed to measure ADAMTS4 (A disintegrin and a metalloproteinase with thrombospondin motifs 4) protease activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough recombinant ADAMTS4, fluorogenic substrate, and assay buffer for 100 enzyme reactionsFigure 1: Illustration of the mechanism behind the ADAMST4 Fluorogenic Assay Kit.ADAMTS4 is incubated with a fluorogenic substrate which is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.Need us to run inhibitor screens or profile your compounds against ADAMTS4? Check out ourProtease Screening Services.