SIRT5 (Sirtuin5) Fluorogenic Assay Kit

The Fluorogenic SIRT5 Assay Kit is a complete assay system designed to measure_x000D_ SIRT5 activity for screening and profiling applications. The Fluorogenic_x000D_ SIRT5 Assay Kit is based on a unique fluorogenic substrate and developer combination._x000D_ This assay method eliminates dealing with the radioactivity, extraction, and_x000D_ chromatography aspects of traditional assays. Using this kit, only three simple steps on_x000D_a microtiter plate are needed to analyze the SIRT5 activity level. First, the Sirtuin 5_x000D_ fluorometric substrate (SIRT substrate 5), containing a succinylated lysine side chain, is_x000D_ incubated with purified SIRT5 enzyme. The desuccinylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_ be measured using a fluorescence reader.