TNKS1 Homogenous Assay Kit

The TNKS1 Homogeneous Assay Kit is designed to measure TNKS1 (tankyrase 1) activity for screening and profiling applications. The TNKS1 Homogeneous Assay Kit comes in a convenient 384-well AlphaLISA® format, with enough purified recombinant TNKS1 (amino acids 1001-1327), biotinylated histone substrate, ADP-Ribose Binding Reagent 1 and assay buffer for 400 enzyme reactions.Figure 1:TNKS1 Homogenous Assay Kit schematic.A sample containing TNKS1 is incubated with a biotinylated histone substrate and NAD+for one hour. This is followed by the addition of acceptor beads and ADP-Ribose Binding Reagent 1, and finally donor beads. Alpha-counts are then measured. Alpha-counts are directly proportional to TNKS1 activity.Need us to run inhibitor screens or profile your compounds against TNKS1? Check out ourPARP/PARPTrap™ Screening ServicesorDNA Replication and Repair Services.