PARP3 Homogenous Assay Kit

The PARP3 Homogeneous Assay Kit is designed to measure PARP3 (poly- (ADP-ribose) polymerase 3) activity for screening and profiling applications. The PARP3 Homogeneous Assay Kit comes in a convenient 384-well AlphaLISA® format, with enough purified recombinant PARP3, biotinylated histone substrate, ADP-Ribose Binding Reagent 1 and assay buffer for 400 enzyme reactions.Figure 1: PARP3 Homogenous Assay Kit schematic.A sample containing PARP3 is incubated with a biotinylated histone substrate and NAD+for one hour. This is followed by the addition of acceptor beads and ADP-Ribose Binding Reagent 1, and finally donor beads. Alpha-counts are then measured. Alpha-counts are directly proportional to PARP3 activity.Need us to run inhibitor screens or profile your compounds against PARP3? Check out ourPARP/PARPTrap™ Screening ServicesorDNA Replication and Repair Screening Services.