PARP1 Homogenous Assay Kit

The PARP1 Homogeneous Assay Kit is designed to measure PARP1 (poly- (ADP-ribose) polymerase 1) activity for screening and profiling applications. The PARP1 Homogeneous Assay Kit comes in a convenient 384-well AlphaLISA® format, with enough purified recombinant PARP1, biotinylated histone substrate, ADP-Ribose Binding Reagent 1 and assay buffer for 400 enzyme reactions.Figure 1: PARP1 Homogenous Assay Kit schematic.A sample containing PARP1 is incubated with a biotinylated histone substrate and NAD+for one hour. This is followed by the addition of acceptor beads and ADP-ribose binding agent, and finally donor beads. Alpha-counts are then measured. Alpha-counts are directly proportional to PARP1 activity.Need us to run inhibitor screens or profile your compounds against PARP1? Check out ourPARP/PARPTrap™ Screening ServicesorDNA Replication and Repair Screening Services.