PARP2 Homogenous Assay Kit

The PARP2 Homogeneous Assay Kit is designed to measure PARP2 (poly- (ADP-ribose) polymerase 2) activity for screening and profiling applications. The PARP2 Homogeneous Assay Kit comes in a convenient 384-well AlphaLISA® format, with enough purified recombinant PARP2 (amino acids 2-583), biotinylated histone substrate, ADP-Ribose Binding Reagent 1 and assay buffer for 400 enzyme reactions.Figure 1: PARP2 Homogenous Assay Kit schematic.A sample containing PARP2 is incubated with a biotinylated histone substrate and NAD+for one hour. This is followed by the addition of acceptor beads and ADP-Ribose Binding Reagent 1, and finally donor beads. Alpha-counts are then measured. Alpha-counts are directly proportional to PARP2 activityNeed us to run inhibitor screens or profile your compounds against PARP2? Check out ourPARP/PARPTrap™ Screening ServicesorDNA Replication and Repair Screening Services.