JMJD2B (KDM4B) Homogenous Assay Kit

The JMJD2B (KDM4B) Homogeneous Assay Kit is designed to measure JMJD2B (Jumonji domain-containing protein 2B) activity for screening and profiling applications. The JMJD2B (KDM4B) Homogeneous Assay Kit comes in a convenient 96-well or 384-well AlphaLISA® format, with enough purified recombinant JMJD2B (amino acids 2-500), substrate, primary antibody, assay and detection buffer for 100 or 400 enzyme reactions.Figure 1: JMJD2B (KDM4B) Homogenous Assay Kit schematic.A sample containing JMJD2B is incubated with a substrate. This is followed by the addition of acceptor beads and primary antibody, and finally donor beads. Alpha-counts are then counted. Alpha-counts are proportional to JMJD2B demethylase activity.Need us to run inhibitor screens or profile your compounds against JMJD2B (KDM4B) ? Check out ourDemethylase Screening Services.