FBXL11 (KDM2A) Homogeneous Assay Kit

The FBXL11 (KDM2A) Homogeneous Assay Kit is designed to measure FBXL11 (F-box and leucine-rich repeat protein 11) activity for screening and profiling applications. The FBXL11 (KDM2A) Homogeneous Assay Kit comes in a convenient 96-well or 384-well AlphaLISA® format, with enough purified recombinant FBXL11 (amino acids 2-700), substrate, primary antibody, assay and detection buffer for 100 or 400 enzyme reactions.Figure 1:FBXL11 (KDM2A) Homogenous Assay Kit schematic.A sample containing FBXL11 is incubated with a biotinylated substrate. This is followed by the addition of acceptor beads and primary antibody, and finally donor beads. Alpha-counts are then counted. Alpha-counts are proportional to FBXL11 demethylase activity