BRD4 (BD2) TR-FRET Assay Kit

The BRD4 (BD2) TR-FRET Assay Kit is designed to measure the inhibition of BRD4_x000D_(BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based_x000D_assay requires no time-consuming washing steps, making it especially suitable for high_x000D_throughput screening applications. The assay procedure is straightforward and simple; a_x000D_sample containing terbium-labeled donor, dye-labeled acceptor, BRD4, substrate, and_x000D_an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured_x000D_using a fluorescence reader.