BRD3 (BD1+BD2) TR-FRET Assay Kit

The BRD3 (BD1+BD2) TR-FRET Assay Kit is designed to measure the inhibition of_x000D_BRD3 (BD1+BD2) binding to its substrate in a homogeneous 384 reaction format. This_x000D_FRET-based assay requires no time-consuming washing steps, making it especially_x000D_suitable for high throughput screening applications. The assay procedure is_x000D_straightforward and simple; a sample containing terbium-labeled donor, dye-labeled_x000D_acceptor, BRD3, ligand, and an inhibitor is incubated for sixty minutes. Then, the_x000D_fluorescence intensity is measured using a fluorescence reader.