BRD4 (BD1+BD2) TR-FRET Assay Kit

The BRD4 (BD1 + BD2) TR-FRET Assay Kit is designed to measure_x000D_the inhibition of BRD4 (BD1 + BD2) binding to its substrate in a homogeneous 384_x000D_reaction format. This FRET-based assay requires no time-consuming washing steps,_x000D_making it especially suitable for high throughput screening applications. The assay_x000D_procedure is straightforward and simple; a sample containing terbium-labeled donor,_x000D_dye-labeled acceptor, BRD4, substrate, and an inhibitor is incubated for sixty minutes._x000D_Then, the fluorescence intensity is measured using a fluorescence reader.