BRD1 TR-FRET Assay Kit

The BRD1 TR-FRET Assay Kit is designed to measure the inhibition of BRD1 binding to its_x000D_substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming_x000D_washing steps, making it especially suitable for high throughput screening_x000D_applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled_x000D_donor, dye-labeled acceptor, BRD1, substrate, and an inhibitor is incubated for 120_x000D_minutes. Then, the fluorescence intensity is measured using a fluorescence reader.