Phospho-ATF2 (T71) Recombinant Monoclonal Antibody

Immunofluorescence staining of A549 cells(treated with 100mM EGF for 20min) with CSB-RA002270A71phHU at 1:63,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).