P53 (Y220C), GST-Tag Recombinant

Recombinant human p53 (tumor protein p53), full length, encompassing amino acids 2-393 (end) . In this construct, the N-terminal end of the protein is fused to GST via a short peptide linker placed between GST consisting of an optimal TEV protease target sequence that allows cleavage of GST (TEV: tobacco etch virus cysteine proteaseBPS Bioscience #50308) . The construct also contains mutation of interest (Y220C) . The recombinant protein was affinity purified.