NutriCulture DMEM, High Glucose

Dulbecco′s Modified Eagle′s Medium (DMEM) is a standard basal cell culture medium that is a modification of Basal Medium Eagle. NutriCulture DMEM contains four-fold concentrations of the amino acids and vitamins than the original Eagle's Minimal Essential Medium. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support the growth of primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, HEK 293, Cos-7, and PC-12. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name DMEM High Glucose. Each lot of NutriCulture DMEM High Glucose is prepared from powdered base medium, tissue culture-grade water, and is sterile filtered using a 0.1 micron filter. Each lot of NutriCulture DMEM High Glucose is tested to confirm the absence of bacterial, fungal, and mycoplasmal contamination.