Notch1 Pathway Reporter Kit (Human)

Notch Pathway Reporter kit is designed for monitoring the activity of the Notch signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for Human NOTCH1 that has a deletion of the entire extracellular domain, leaving the transmembrane and intracellular domains intact (Notch1DE). Inside the cells, the NOTCH1 DE is cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_This kit contains the expression vector for Human Notch1DE. We also offer the Mouse Notch1DE expression vector (BPS Bioscience #60509), as well as a Mouse Notch1 NICD expression vector, which is constitutively active without processing by γ-secretase (BPS Bioscience #79504)._x000D_