Multiplex ELISA Kit For Human Malaria (5-Plex)

<h2>Product Overview</h2>_x000D_<p>The Multiplex ELISA Kit For Human Malaria (5-Plex) is a robust and fully quantitative chemiluminescent assay for the concurrent surveillance of four malaria biomarkers. The assay simultaneously measures HRP2, LDH-Pan, LDH-Pv, LDH-Pf, and one host biomarker, CRP. The monitoring of subclinical Malaria infections and prevalence of HRP2-deletions is achieved by the coupling of the most sensitive commercially available HPR2 assay with LDH detection. Validated protocols for whole blood samples are in place, with protocols for dry blood spots in development.</p><p>Each kit contains a 96-well plate and all other reagents necessary to perform the assay. The individual wells of the plate are precisely and reproducibly arrayed with each of the five biomarkers for accurate results.</p><p>In 3.5 hours, each kit will report quantities of 80 samples for the four Malaria biomarkers and one host biomarker CRP. This is achieved while only using 15 μl of whole blood per sample.</p>_x000D__x000D_<h2>Assay Ranges</h2>_x000D_<table class="w-100 table table-striped"><thead class="bg-grey text-white"><tr><th>Analyte</th><th>Assay Type</th><th>Calibrator Range</th><th>Upper Limit of Quantification (ULOQ)</th><th>Lower Limit of Quantification (LLOQ)</th><th>Limit of Detection</th></tr></thead><tbody><tr><td>CRP</td><td>Competitive</td><td>62,800 – 61 (ng/mL)</td><td>11,125 (ng/mL)</td><td>60 (ng/mL)</td><td>30.5 (ng/mL)</td></tr><tr><td>HRP2</td><td>Sandwich</td><td>950 – 0.9 (pg/mL)</td><td> 675 (pg/mL)</td><td> 0.92 (pg/mL)</td><td> 0.45 (pg/mL)</td></tr><tr><td>LDH -Pan</td><td>Sandwich</td><td> 70,750 – 69 (pg/mL)</td><td> 17,687.5 (pg/mL)</td><td> 24 (pg/mL)</td><td> 34.5 (pg/mL)</td></tr><tr><td>LDH-Pf</td><td>Sandwich</td><td>22,500 - 22 (pg/mL)</td><td> 5,750 (pg/mL)</td><td> 8 (pg/mL)</td><td>11 (pg/mL)</td></tr><tr><td>LDH-Pv</td><td>Sandwich</td><td>43,750 – 43 (pg/mL)</td><td>5,000 (pg/mL)</td><td>6 (pg/mL)</td><td>21.5 (pg/mL)</td></tr></tbody></table>_x000D__x000D_<h2>Kit Components</h2>_x000D_<p>Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.</p>_x000D__x000D_<p>Reagents include:</p>_x000D_<p>Wash Buffer Concentrate (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;_x000D_Assay Diluent: Liquid, 6 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives;_x000D_Calibrator Diluent: Lyophilized, buffered protein solution containing preservatives;_x000D_Competitor: Lyophilized, biotinylated competitive antigen;_x000D_Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives;_x000D_Calibrator: Lyophilized, recombinant antigens in a buffered protein base;_x000D_Streptavidin-HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;_x000D_Substrate A+: Liquid, 3 mL/vial of stabilized hydrogen peroxide;_x000D_Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer;_x000D_Plate Seals (3): Adhesive strips</p>