Multiplex ELISA Kit For Mouse Cytokine - Inflammation (13-plex)

<h2>Product Overview</h2>_x000D_<p>The Multiplex Mouse Cytokine &ndash; Inflammation (13-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 13 biomarkers or analytes. Using just 25 µl of sample per well, up to 80 samples can be assayed for all 13 markers in the panel within 5.5 hours.</p><p>The Multiplex Mouse Cytokine &ndash; Inflammation (13-plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 13 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.</p> _x000D_<h2>Assay Ranges</h2>_x000D_<table class="w-100 table table-striped">_x000D_ <thead class="bg-grey text-white"><tr><th>Analyte</th><th>Assay Type</th><th>Calibrator Range</th><th>Upper Limit of Quantification (ULOQ)</th><th>Lower Limit of Quantification (LLOQ)</th><th>Limit of Detection</th></tr></thead>_x000D_ <tbody>_x000D_ <tr><td>GMCSF</td><td>Sandwich</td><td>560 - 0.77 (pg/mL)</td><td>392 (pg/mL)</td><td>1 (pg/mL)</td><td>0.38 (pg/mL)</td></tr>_x000D_ <tr><td>IL-1α</td><td>Sandwich</td><td>610 - 0.84 (pg/mL)</td><td>427 (pg/mL)</td><td>1.1 (pg/mL)</td><td>0.42 (pg/mL)</td></tr>_x000D_ <tr><td>IL-1β</td><td>Sandwich</td><td>2500 - 3.43 (pg/mL)</td><td>1750 (pg/mL)</td><td>4.5 (pg/mL)</td><td>1.71 (pg/mL)</td></tr>_x000D_ <tr><td>IL-2</td><td>Sandwich</td><td>360 - 0.49 (pg/mL)</td><td>252 (pg/mL)</td><td>0.6 (pg/mL)</td><td>0.25 (pg/mL)</td></tr>_x000D_ <tr><td>IL-3</td><td>Sandwich</td><td>60 - 0.08 (pg/mL)</td><td>42 (pg/mL)</td><td>0.1 (pg/mL)</td><td>0.04 (pg/mL)</td></tr>_x000D_ <tr><td>IL-4</td><td>Sandwich</td><td>400 - 0.55 (pg/mL)</td><td>280 (pg/mL)</td><td>0.7 (pg/mL)</td><td>0.27 (pg/mL)</td></tr>_x000D_ <tr><td>IL-6</td><td>Sandwich</td><td>504 - 0.69 (pg/mL)</td><td>353 (pg/mL)</td><td>0.91 (pg/mL)</td><td>0.35 (pg/mL)</td></tr>_x000D_ <tr><td>IL-10</td><td>Sandwich</td><td>667 - 0.91 (pg/mL)</td><td>467 (pg/mL)</td><td>1.33 (pg/mL)</td><td>0.46 (pg/mL)</td></tr>_x000D_ <tr><td>IL-12p70</td><td>Sandwich</td><td>572 - 0.78 (pg/mL)</td><td>400 (pg/mL)</td><td>1.01 (pg/mL)</td><td>0.39 (pg/mL)</td></tr>_x000D_ <tr><td>IL-17A</td><td>Sandwich</td><td>760 - 1.04 (pg/mL)</td><td>532 (pg/mL)</td><td>1.4 (pg/mL)</td><td>0.52 (pg/mL)</td></tr>_x000D_ <tr><td>MIP-1α</td><td>Sandwich</td><td>1000 - 1.37 (pg/mL)</td><td>700 (pg/mL)</td><td>1.8 (pg/mL)</td><td>0.69</td></tr>_x000D_ <tr><td>RANTES</td><td>Sandwich</td><td>1653 - 2.27 (pg/mL)</td><td>1157 (pg/mL)</td><td>2.92 (pg/mL)</td><td>1.13</td></tr>_x000D_ <tr><td>TNFα</td><td>Sandwich</td><td>429 - 0.59 (pg/mL)</td><td>300 (pg/mL)</td><td>0.69 (pg/mL)</td><td>0.29</td></tr>_x000D_ </tbody>_x000D_</table><h2>Kit Components</h2>_x000D_<p>Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format, please contact us for customization.</p>_x000D_<p>Reagents Include:</p>_x000D_<p>Calibrator: Lyophilized, recombinant antigens in a buffered protein base;Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives ;Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide ;Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer ;Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives ;Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;Plate Seals (3): Adhesive strips</p>