MSK1(Phospho Thr581) Polyclonal Antibody

Catalytic activity:ATP + a protein = ADP + a phosphoprotein.|cofactor:Magnesium.|enzyme regulation:Appears to be activated by multiple phosphorylations on threonine and serine residues. ERK1/2 and MAPK14/p38-alpha may play a role in this process.|Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics| stress stimuli and epidemal growth-factor (EGF)| which results in the transcriptional activation of several immediate early genes| including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).|miscellaneous:Enzyme activity requires the presence of both kinase domains.|PTM:Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain| and their phosphorylation is essential for the Catalytic activity of the N-terminal kinase domain.|Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.|Contains 1 AGC-kinase C-terminal domain.|Contains 2 protein kinase domains.|subcellular location:Predominantly nuclear. Partially cytoplasmic.|subunit:Forms a complex with either ERK1 or ERK2 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA5 associates with and phosphorylates the NF-kappa-B p65 subunit RELA.|tissue specificity:Widely expressed with high levels in heart| brain and placenta. Less abundant in lung| kidney and liver.|