KRAS (G12C) Coupled Nucleotide Exchange Assay Kit

The KRAS (G12C) Coupled Nucleotide Exchange Assay Kit is designed for screening and profiling of KRAS (G12C) antagonists/inhibitors by monitoring the binding of an effector protein such as the Ras binding domain of Raf1, (RBD-cRaf) to KRAS (G12C) . With this kit, a few simple steps on a microtiter plate are required for nucleotide exchange detection. First, a sample containing GDP-loaded KRAS (G12C) is incubated with SOS1 and GTP for the nucleotide exchange. Next, RBD-cRAF is added and incubated for the effector-RAS binding. Then, acceptor and donor beads are added and incubated for detection followed by reading the Alpha-counts.SOS1 (son of sevenless) is a guanine nucleotide exchange factor that facilitates the exchange of GDP for GTP. GDP-loaded KRAS (G12C) is in an inactive state, and does not interact with the Ras-binding domain (RBD) of cRAF.  SOS1 assists in the release of GDP from KRAS (G12C) so that GTP can occupy the nucleotide binding pocket. This results in a conformational change in KRAS (G12C) that permits its binding to RBD-cRAF. The KRAS (G12C) Coupled Nucleotide Exchange Assay Kit utilizes GST-tagged RBD-cRAF and His-tagged KRAS (G12C) to assay binding of KRAS (G12C) to RBD-cRAF in the Alpha assay. Glutathione acceptor and Ni chelate donor beads are brought into proximal range by the binding of KRAS (G12C) and RBD-cRAF, enabling the energy transfer from the donor to acceptor beads after laser excitation.Figure 1: Illustration of the assay principle.