Histone H4 (R3) Methyltransferase Assay Kit

The Histone H4 (R3) Universal Methyltransferase Assay Kit is designed for the detection of histone H4 (R3) methyltransferase (HMT) activity using purified enzymes or cell extracts. Histone H4 (R3) methyltransferases are enzymes that catalyze the transfer of a methyl group from the cofactor S-adenosylmethionine to arginine 3 residue of histone H4.The key to the Histone H4 (R3) Universal Methyltransferase Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.