Histone H3 (K9) Universal Methyltransferase Assay Kit

The Histone H3 (K9) Universal Methyltransferase Assay Kitis designed to measure Histone H3 (K9) methyltransferase activity for screening and profiling applications. This kit comes in a convenient format, with a plate precoated with histone H3 peptide substrate, recombinant human G9a enzyme (amino acids 785-1210), SUV39H1 (amino acids 82-412) and SUV39H2 (amino acids 26-350), high specific antibody against the methylated lysine residue of Histone H3, secondary HRP-labeled antibody, S-adenosylmethionine and Methyltransferase Assay Buffer for 100 enzyme reactions.Figure 1: Histone H3 (K9) Universal Methyltransferase Assay Kit workflow diagram.First, S-adenosylmethionine is incubated with the assay buffer, methyltransferase enzyme, and the inhibitor of choice for one hour in a histone-precoated plate. This is followed by the addition of a primary antibody against Lys (Me) . Addition of a chemiluminescent HRP substrate provides a luminescence signal, which directly correlates with the activity of G9a.This product has beencited 1 time.