Caspase-7 Homogeneous Assay Kit

Homogeneous Caspase-7 Assay Kit is a kit designed to measure caspase-7 activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough recombinant caspase-7, fluorogenic substrate, and assay buffer for 100 enzyme reactions. This kit also contains Ac-DNLD-CHO, a potent caspase-7 inhibitor, as control.Figure 1: Illustration of the mechanism behind the Homogeneous Caspase-7 Assay Kit.The substrate Ac-DEVD-AFC is incubated with caspase-7. Proteolysis of the substrate releases the AFC fluorophore. Fluorescence intensity can be measured with a microplate reader able to read fluorescence with λexc=400 nm and λem = 505 nm.   Fluorescence is thus proportional to caspase-7 activity.