Caspase-3 Homogeneous Assay Kit

The Homogeneous Caspase-3 Assay Kit is designed to measure caspase-3 activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough recombinant caspase-3, fluorogenic substrate, and assay buffer for 100 enzyme reactions. This kit also contains Ac-DNLD-CHO, a potent caspase-3 inhibitor, as control.Figure 1: Illustration of the mechanism behind the Homogeneous Caspase-3 Assay Kit.The fluorogenic substrate Ac-DEVD-AFC is incubated with caspase-3. Proteolysis of the fluorogenic substrate releases the AFC fluorophore. Fluorescence intensity can be measured with a microplate reader able to read fluorescence at λexc=400 nm and λem = 505 nm. Fluorescence is thus proportional to caspase-3 activity.