BCL-2 TR-FRET Assay Kit

The BCL-2 TR-FRET Assay Kit is designed to measure the inhibition of BCL-2_x000D_binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no_x000D_time-consuming washing steps, making it especially suitable for high throughput screening_x000D_applications. The assay procedure is straightforward and simple; a sample containing terbiumlabeled_x000D_donor, dye-labeled acceptor, BCL-2, peptide ligand, and an inhibitor is incubated for 120_x000D_minutes. Then, the fluorescence intensity is measured using a fluorescence reader.