TAF1L (BD2) Inhibitor Screening Assay Kit

The TAF1L (BD2) Inhibitor Screening Assay Kit is designed to measure the inhibition of TAF1L binding to its substrate. The key to the assay kit is specific binding of the TAF1L bromodomain 2 (a.a. 1517-1649) to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing TAF1L and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.