Sortase A Assay Kit

The Sortase A Assay Kit is a fluorogenic assay kit designed to measure Sortase A proteolytic activity for screening and profiling applications.Figure 1: Illustration of the assay principle.The fluorescence emitted by donor DNP is quenched due to the proximity of the Abz acceptor in the intact peptide. The protease cleaves the peptide between T (threonine) and G (glycine) and forms an intermediate complex between the cysteine residue within the active site and the C-terminus of the peptide substrate.The cleaved substrateis then covalently boundedto the triglycine nucleophile. The glycine-DNP fragment is released, freeing the emission of fluorescence by DNP.Need us to run inhibitor screens or profile your compounds against Sortase A? Check out ourProtease Screening Services.