Sialidase Fluorogenic Assay Kit

The Sialidase Fluorogenic Assay Kitis designed to measure Sialidase NANH (N-acylneuraminate glycohydrolase) activity for screening and profiling applications. It comes in a convenient 96-well format, with purified recombinantSalmonella typhimuriumsialidase, Sialidase substrate, Sialidase assay buffer, and Stop Solution for 100 enzyme reactions. Sialidase inhibitor DANA (N-Acetyl-2,3-dehydro-2-deoxyneuraminic acid) is also included as a control.The Sialidase fluorogenic substrate is incubated with Sialidase. Hydrolyzation of the Sialidase substrate releases a fluorophore (λexc/λem = 365 nm/445 nm) . The increase in fluorescence at λ=445 nm is directly proportional to Sialidase activity.Need us to run inhibitor screens or profile your compounds against Sialidase? Check out ourMetabolic Enzyme Screening Services.