SetDB1 Chemiluminescent Assay Kit

The SetDB1 Chemiluminescent Assay Kit is designed to measure SetDB1_x000D_activity for screening and profiling applications. The key to the SetDB1 Direct Activity Assay Kit is a highly specific antibody that_x000D_recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are_x000D_required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample_x000D_containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally,_x000D_the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP_x000D_substrate to produce chemiluminescence that can then be measured using a chemiluminescence_x000D_reader.