SCRIPTUM-37 RT, 10.000 Units

SCRIPTUM-37 Reverse Transcriptase (RT) (200 U/µl) is an M-MuLV reverse transcriptase that rapidly and specifically generates cDNA. The appropriate reaction temperature for reverse transcription depends on the structural properties of the RNA. The optimum temperature of the SCRIPTUM-37 RT is 37°C and is therefore ideally suitedfor routine applications with simple RNA templates. A higher temperature (maximum 55°C) should be used for highly structured RNA templates. Here we recommend SCRIPTUM-55 RT or SCRIPTUM-60 RT. For best results, our SCRIPTUM-37 Reverse Transcriptase is offered with aspecifically optimised, 5x concentrated reaction buffer.PropertiesFor the synthesis of cDNA molecules (100 bp - 7 kb) Particularly suitable for routine applications with simple RNA templatesOptimum temperature = 37°CGood to knowSingle-stranded nucleic acids often form intramolecular double strands through hydrogen bonds between complementary sections. This results in secondary structures with so-called ‘stems’ (paired double-stranded sections) and ‘loops’ (double-stranded sections that include single-stranded sections) . RNA secondary structures can take various forms, such as hairpin loops, interior loops or multi-loops.