PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit

The PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit is an ELISA-based assay  designed to measure the binding between PD-1 (programmed cell death 1) and PD-L1 (programmed death-ligand 1) protein for screening and profiling applications. This kit comes with enough purified PD-1 (amino acids 25-167) and biotinylated PD-L1 (amino acids 19-239), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.A 96-well plate is coated with PD-1 protein. After coating and blocking, biotinylated PD-L1is added in an optimized assay buffer. Next, unbound biotinylated PD-L1 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of PD-L1 binding to PD-1.Need us to run inhibitor screens or profile your compounds? Check out ourImmunotherapy Biochemical Screening Services.This product has beencited 1 time.