PD-1:PD-L2[Biotinylated] Inhibitor Screening Colorimetric Assay Kit

Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand_x000D_attenuates immune responses and is exploited by both tumors and viruses. The PD-1:PDL2[_x000D_Biotinylated] Inhibitor Screening Colorimetric Assay Kit is designed for screening inhibitors of_x000D_PD-L2 ligand binding to PD-1 receptor. The key to this kit is the high affinity of biotin-labeled PD-L2 for streptavidin-HRP._x000D_Only a few simple steps on a microtiter plate are required for the assay. First, PD-1 is coated on_x000D_a 96-well plate. Next, PD-L2 is incubated with PD-1 on the plate. Finally, the plate is treated with_x000D_streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which_x000D_can then be measured using a UV/Vis spectrophotometer microplate reader.