PD-1[Biotinylated]:PD-L2 Inhibitor Screening Assay Kit

The PD-1[Biotinylated]:PD-L2 Inhibitor Screening Assay Kit is an ELISA-based assay designed to measure the binding between PD-1 (programmed cell death 1) and PD-L2 (programmed death-ligand 2) protein for screening and profiling applications. This kit comes with enough purified biotinylated PD-1 (amino acids 25-167) and PD-L2 (amino acids 20-219), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.Figure 1. PD-1[Biotinylated]:PD-L2 Inhibitor ScreeningAssay Kit schematic.A 96-well plate is coated with PD-L2 protein. After coating and blocking, biotinylated PD-1 is added in an optimized assay buffer. Next, unbound biotinylated PD-1 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of PD-1 binding to PD-L2Need us to run inhibitor screens or profile your compounds against PD-1[Biotinylated]:PD-L2? Check out ourImmunotherapy Biochemical Screening Services.