OxiSelect Aldehyde-Induced DNA Damage ELISA Combo Kit (Ethenoadenosine / Ethenocytidine Quantitation)

The OxiSelect Aldehyde-Induced DNA Damage ELISA Combo Kit (Ethenoadenosine / Ethenocytidine Quantitation) is a competitive ELISA kit allowing the quantitation of ethenoadenosine or ethenocytidine adducts in DNA isolated from cells or tissues. Samples and standards are added to a provided microplate preabsorbed with etheno-damaged DNA. A primary antibody is added and binds competitively to ethenoadenosine in the unknown sample or to the etheno adduct in the DNA coated on the plate. After washing a secondary antibody is added. A low signal means most of the primary antibody was bound to the unknown, indicating a high level of etheno adducts in the sample. Each combo kit can perform up to 96 assays combined for ethenoadenosine and ethenocytidine. The two targets are assayed in separate wells, but any well on the provided plate may be used for either target in any ratio (e.g. 64 wells for ethenoadenosine and 32 wells for ethenocytidine, or 48 wells for each).