Optic Nerve Lysate

Human optic nerve tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human optic nerve tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the optic nerve tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The optic nerve tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.