NOSIP ELISA Kit (Human) (OKCD00235)

Description of target: E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (By similarity). Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity (PubMed:11149895, PubMed:15548660, PubMed:16135813).By similarity&#xd; &lt;p>Manually curated information which has been propagated from a related experimentally characterized protein.&lt;/p>&#xd; &#xd; &#xd; &lt;p>&lt;a href="/manual/evidences#ECO:0000250">More…&lt;/a>&lt;/p> Manual assertion inferred from sequence similarity toiUniProtKB:Q9D6T0&nbsp;(NOSIP_MOUSE)3 Publications&#xd; &lt;p>Manually curated information for which there is published experimental evidence.&lt;/p>&#xd; &#xd; &#xd; &lt;p>&lt;a href="/manual/evidences#ECO:0000269">More…&lt;/a>&lt;/p> Manual assertion based on experiment iniRef.1"NOSIP, a novel modulator of endothelial nitric oxide synthase activity."_x005F_x005F_x000D_Dedio J., Koenig P., Wohlfart P., Schroeder C., Kummer W., Mueller-Esterl W._x005F_x005F_x000D_FASEB J. 15:79-89(2001) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH NOS3, FUNCTION.Ref.4"Nitric oxide synthase (NOS)-interacting protein interacts with neuronal NOS and regulates its distribution and activity."_x005F_x005F_x000D_Dreyer J., Schleicher M., Tappe A., Schilling K., Kuner T., Kusumawidijaja G., Mueller-Esterl W., Oess S., Kuner R._x005F_x005F_x000D_J. Neurosci. 24:10454-10465(2004) [PubMed] [Europe PMC] [Abstract]Cited for: INTERACTION WITH NOS1, SUBCELLULAR LOCATION, FUNCTION.Ref.5"Cell cycle-regulated inactivation of endothelial NO synthase through NOSIP-dependent targeting to the cytoskeleton."_x005F_x005F_x000D_Schleicher M., Brundin F., Gross S., Mueller-Esterl W., Oess S._x005F_x005F_x000D_Mol. Cell. Biol. 25:8251-8258(2005) [PubMed] [Europe PMC] [Abstract]Cited for: SUBCELLULAR LOCATION, FUNCTION. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.053 ng/mL