Monkey PD-1[Biotinylated]:PD-L1 Inhibitor Screening Assay Kit

The Monkey PD1[Biotinylated]: PD-L1 Inhibitor Screening Assay Kit is a chemiluminescence assay designed for screening and profiling molecules that block the binding of PD-1 (programmed  cell death protein 1) to PD-L1 (programmed cell death 1 ligand 1) . This assay kit comes in a convenient 96-well format, with enough purified monkey biotin-labeled PD-1 (amino acids 25-167), monkey PD-L1 (amino acids 25-167), assay buffers and detection reagents for 100 enzyme reactions.The key to this kit is the high affinity of biotin-labeled PD-1 for streptavidin-HRP. First, a 96-well plate is coated with PD-L1, followed by incubation with PD-1. Finally, the plate is treated with streptavidin-HRP followed by addition of HRP substrate. The chemiluminescence signal can be detected with a microplate reader capable of reading chemiluminescence. The signal generated is proportional to the binding between PD-1 and PD-L1.