MAGIC RED CATHEPSIN B KIT (OKSA11290)

Description of target: Cathepsin Magic Red^TM kits measure cathepsin B protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product. The Magic Red^TM reagent contains a cathepsin B target sequence peptide (RR)2 linked to a red_x000D_(Cresyl Violet) fluorescent probe. The Cathepsin Magic Red^TM kits are suitable for cells in suspension, adherent cells, thin frozen tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits. This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates._x000D_;Species reactivity: Human;Application: IF;Assay info: ;Sensitivity: