IDO1 Fluorogenic Inhibitor Screening Assay Kit

TheIDO1Fluorogenic Inhibitor Screening Assay Kitis designed to measure enzyme inhibition of indoleamine 2,3-dioxygenase 1 (IDO1) . TheIDO1 Fluorogenic Inhibitor Screening Assay Kitis simple to use and detects fluorescence at long wavelengths, which minimizes potential errors due to compound interference. In the assay, the inhibitor and enzyme are added to a sample containing L-Trp substrate. After a 1 hourincubation at room temperature, the fluorescence solution is added and incubated at 37oC for four hours. Activity is measured by reading sample fluorescence at l=510 nm following excitation of the reaction product at l=400 nm.