HighQC™ Human IPS Cell Line (Episomal, HFF)

Footprint-free human iPS (induced pluripotent stem) cell line derived from human foreskin fibroblasts (HFFs) by ectopic expression of OCT4, SOX2, KLF4, and c-MYC genes using episomal plasmids. The cells were derived using morphological selection criteria and without the use of fluorescent marker or drug selection. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells are identical to that of human ES cells. The cells also express the pluripotency markers SSEA-3 and Nanog, and demonstrate strong endogenous alkaline phosphatase. High viability, low passage iPS cells have been pre-adapted to serum-free, feeder-free culture conditions.