Fluorescent Dopamine Neurotransmitter Ligand

Pharmalogical properties virtually identical to native dopamine. DnsylD-1 operates selectively in uptake and binding assays at nanomolar concentrations.  The native dopamine chemical structure is the major component of this fluorescent catecholamine, generating a fluorescent neurotransmitter molecule with high affinity toward all dopamine receptor subtypes as well as to the dopamine transporter. The small dansyl tag with a 5-carbon polylinker to dopamine generates a stable, bright fluorescent signal with negligible steric hindrance. The dansyl fluorescent tag offers a large stokes shift that minimizes autofluorescence and non-specific background signals:  (Ex/Em:  313/515nm). The fluorescent signal of DnsylD-1TM is blockable by standard inhibitors to dopamine receptors and DAT. Standard inhibitors can be employed to distinguish the interaction of DnsylD-1TM with dopamine receptors and DAT. Fluorescence from DnsylD-1TM colocalizes with tyrosine hydroxylase and identifies dopaminergic neurons. To assay for dopamine receptor binding, first suspend the provided solid in DMSO (10 - 50 µl).  Centrifuge to clarify, and collect the DMSO soluble fraction, which can be aliquoted and stored at -20oC:  DnsylD-1TM when suspended in DMSO and frozen at -20oC is stable for at least 2 years.  Dilute the DMSO solubilized material into your assay buffer (for example PBS-BSA) to the desired assay concentration.  Detect DnsylD-1TM by fluorescent microscopy, with a fluorescent