![DIASpot Polymyositis/Scleroderma8 IgG (Autoimmune Multiplex Dot Assay Kit) [CE]](/images/no-image-product-box.jpg)
The test is based on the principle of an Enzyme Immunoassay. The test strip is composed of a membrane fixed on a plastic support. During test procedure, the strips are incubated with diluted patients’ sera. Human antibodies, if present, bind to the corresponding specific antigen(s) on the membrane. Unbound or excess antibodies are removed by washing and AP-conjugated goat antibodies against human IgG are added to the strips. This enzyme conjugate binds to the antigen-antibody complexes. After a
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