CTLA4 [Biotinylated]:B7-1 Inhibitor Screening Chemiluminescence Assay Kit

The CTLA4 [Biotinylated]:B7-1 Inhibitor Screening Chemiluminescence Assay Kit  is an ELISA-based assay designed to measure the binding between CTLA4 (cytotoxic T-lymphocyte associated protein 4) and B7-1 (or CD80) protein for screening and profiling applications. This kit comes with enough purified CTLA4 (amino acids 36-162) and biotinylated B7-1 (amino acids 35-242), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.Figure 1. CTLA4 [Biotinylated]:B7-1 Inhibitor Screening Chemiluminescence Assay Kit schematic.A 96-well plate is coated with B7-1 protein. After coating and blocking, biotinylated CTLA4 is added in an optimized assay buffer. Next, unbound biotinylated CTLA4 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of CTLA4 binding to B7-1.Interested in having us perform the screening and profiling of inhibitors or blocking antibodies? Visit ourImmunotherapy Biochemical Screening Services.