Canine Alveolar Epithelial Cells

Canine Primary Alveolar Epithelial Cells are isolated from alveolar tissue of beagle dog. Canine Primary Alveolar Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Culture Complete Growth Medium for 3-5 days. Prior to shipping, cells at passage 2 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods.