Anti-Phospho-Smad3 (S425) antibody

Smad3 is a protein encoded by the SMAD3 gene which is approximately 48 kDa. Smad3 is localised to the cytoplasm and nucleus. It is involved in SMAD2/3 MH2 domain mutants in cancer, the cell cycle and toll-like receptor signalling pathway. It an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. It binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Smad3 is expressed in the nervous system, pancreas, lung, kidney and intestine. Mutations in the SMAD3 gene may result in colorectal cancer and Loeys-Dietz syndrome. STJ90745 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous Smad3 protein which only binds about S425 when S425 is phosphorylated.