Aflatoxin Competitive ELISA Kit

Cell Biolabs? Aflatoxin Competitive ELISA Kit provides a convenient method for the detection of total Aflatoxin B1 and Aflatoxin B2 adducts in protein samples. First, an AFB1 conjugate is coated on an ELISA plate.  The unknown AFB1/2 samples or AFB1-BSA standards are then added to the AFB1 conjugate preabsorbed ELISA plate.  After a brief incubation, an anti-AFB1/2 monoclonal antibody is added, followed by an HRP conjugated secondary antibody.  The total content of AFB1 plus AFB2 in unknown samples is determined by comparison with a predetermined AFB1-BSA standard curve.